Detection and Automated Scoring of Dicentric Chromosomes in Nonstimulated Lymphocyte Prematurely Condensed Chromosomes After Telomere and Centromere Staining
نویسندگان
چکیده
منابع مشابه
Telomere Disruption Results in Non-Random Formation of De Novo Dicentric Chromosomes Involving Acrocentric Human Chromosomes
Genome rearrangement often produces chromosomes with two centromeres (dicentrics) that are inherently unstable because of bridge formation and breakage during cell division. However, mammalian dicentrics, and particularly those in humans, can be quite stable, usually because one centromere is functionally silenced. Molecular mechanisms of centromere inactivation are poorly understood since ther...
متن کاملdeveloping an automated cytogenetic imaging system for detection of dicentric chromosomes in biological dosimetry
background: high-energy ionizing radiation is harmful and changes the genetic makeup of dna, which can lead to increased risk of cancer. thus, the exposure of radiation dose should be under control and limited. ionizing radiation might lead to some chromosome aberrations like dicentric. there is a strong relation between the frequency of dicentric chromosome in metaphase, and the received dose....
متن کاملEpigenetic Inactivation and Subsequent Heterochromatinization of a Centromere Stabilize Dicentric Chromosomes
BACKGROUND The kinetochore is a multiprotein complex that forms on a chromosomal locus designated as the centromere, which links the chromosome to the spindle during mitosis and meiosis. Most eukaryotes, with the exception of holocentric species, have a single distinct centromere per chromosome, and the presence of multiple centromeres on a single chromosome is predicted to cause breakage and/o...
متن کاملTriage biodosimetry using centromeric/telomeric PNA probes and Giemsa staining to score dicentrics or excess fragments in non-stimulated lymphocyte prematurely condensed chromosomes.
The frequency of dicentric chromosomes in human peripheral blood lymphocytes at metaphase is considered as the "gold-standard" method for biological dosimetry and, presently, it is the most widely used for dose assessment. Yet, it needs lymphocyte stimulation and a 2-day culture, failing the requirement of rapid dose estimation, which is a high priority in radiation emergency medicine and triag...
متن کاملHigh frequency of centromere inactivation resulting in stable dicentric chromosomes of maize.
Somatic chromosome spreads from maize (Zea mays L.) plants containing B-A translocation chromosomes undergoing the chromosome type breakage-fusion-bridge cycle were examined by FISH. The size and type of extra chromosomes varied among cells of the same individual. A collection of minichromosomes derived from the chromosome type breakage-fusion-bridge cycle was examined for the presence of stabl...
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ژورنال
عنوان ژورنال: International Journal of Radiation Oncology*Biology*Physics
سال: 2015
ISSN: 0360-3016
DOI: 10.1016/j.ijrobp.2014.10.048